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Objective To discuss the changes of serum gastric cancer associated antigen MG7 (MG7-Ag),carbohydrate antigen 125 (CA125) levels before and after chemotherapy in patients with advanced cervical cancer and their relationships with the curative effect.Methods A total of 150 advanced cervical cancer patients with paclitaxel combined with cisplatin treatment were selected from June 2014 to June 2017 in our hospital,and 150 cases of healthy persons were selected at the same time.The serum MG7-Ag and CA125 levels of all persons were detected.The differences of MG7-Ag and CA125 levels between healthy persons and advanced cervical cancer patients were analyzed.And the changes of MG7-Ag,CA125 levels before and after chemotherapy and the relationships between the MG7-Ag,CA125 levels and the curative effect of chemotherapy were analyzed.Results The serum MG7-Ag and CA125 levels in the patients with advanced cervical cancer were significantly higher than those in the healthy persons [(15.36 ±2.23) U/ml vs.(2.42 ±0.31) U/ml,(275.42 ± 30.52) U/ml vs.(1 1.89 ± 1.77) U/ml],and the differences were statistically significant (t =26.215,P ＜0.001;t =82.243,P ＜ 0.001).After chemotherapy for 150 patients with advanced cervical cancer,the complete remission (CR) was 5 cases (3.33％),the partial remission (PR) was 58 cases (38.67％),the stable disease (SD) was 60 cases (40.00％),and the progression disease (PD) was 27 cases (18.00％).The MG7-Ag and CA125 levels after chemotherapy in the patients with CR and PR were decreased significantly than those before chemotherapy [(7.12 ± 1.22) U/ml vs.(15.12 ± 3.32) U/ml,(180.34±22.42) U/mlvs.(274.21 ±30.82) U/ml,(9.22 ±1.77) U/mlvs.(15.52 ±3.53) U/ml,(206.75 ±23.02) U/ml vs.(276.12 ±30.92) U/ml],and the differences were statistically significant (t =5.057,P=0.001;t=12.150,P＜0.001,t =5.507,P＜0.001;t =13.705,P＜0.001).The MG7-Ag and CA125 levels after chemotherapy in the patients with PD were increased significantly than those before chemotherapy [(24.21 ± 3.64) U/ml vs.(15.56 ± 3.87) U/ml,(376.64 ± 45.12) U/ml vs.(280.75 ± 31.88) U/ml],and the differences were statistically significant (t =8.460,P ＜0.001;t =9.019,P ＜0.001).The MG7-Ag,CA125 levels after chemotherapy in the patients with SD were increased than those before chemotherapy [(16.01±2.37) U/mlvs.(15.67±3.55) U/ml,(285.26±37.34) U/mlvs.(277.04±31.73) U/ml],but the differences were not statistically significant (t =0.617,P =0.538;t =1.299,P =0.196).The results of receiver operatingcharacteristic (ROC) curve analysis showed that,in the aspect of sensitivity,specificity and accuracy of chemotherapy effect evaluation for cervical cancer,MG7-Ag decline percentage with ＞ 25％ as the critical value were 82.54％,82.76％,82.67％,CA125 decline percentage with ＞ 18％ as the critical value were 85.71％,86.21％,86.00％,and the combination of the two were 96.83％,97.70％,97.33 ％.The combination of the two were significantly higher than that of the two alone,and the differences were statistically significant (x2 =6.948,P =0.008;x2 =11.018,P =0.001;x2 =17.926,P ＜0.001;x2 =4.881,P =0.027;x2 =7.768 P =0.005;x2 =12.611,P ＜ O.001).Conclusion The serum MG7-Ag and CA125 levels in patients with advanced cervical cancer are higher than those in healthy persons.The MG7-Ag and CA125 levels of patients with CR and PR are decreased after chemotherapy,and their changes of serum levels can be used as the important indexes for evaluating the efficacy of chemotherapy.The combined detection of them has the better evaluation efficiency,and it is worth for further clinical promotion.
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Objective To explore a highly sensitive and highly specific method to detect the serum MG7 antigen (Ag) level for early gastric cancer diagnosis.Methods The serum MG7-Ag level was detected by enzyme-linked immunosorbent assay (ELISA) method in 116 preoperative gastric cancer patients,63 postoperative gastric cancer patients,41 patients with precancerous lesion,37 patients with precancerous diseases,50 healthy individuals and 281 patients with other cancers.Meanwhile,the expression of MG7-Ag was also examined with immunohistochemistry in patients with gastric cancer or precancerous lesion.Chi-square test was used for comparing positive rates of the two detection methods.Results The positive rate of MG7-Ag determined by ELISA was 83.6％(97/116) of preoperative gastric cancer patients,45.2％(28/62) of lung cancer patients,45.5％(20/44) of rectal cancer patients,17.6％ (12/68) of colonic cancer patients,14.2％ (6/42) of breast cancer patients,47.6％ (30/63) of postoperative gastric cancer patients,19.5 ％ (8/4 1) of patients with precancerous lesions,5.4 ％ (2/37) of patients with precancerous diseases and 0 of healthy individuals.The sensitivity of ELISA (83.6 ％) was similar with that of immunohistochemistry (94.0％)(P＞0.05).However,the false positive rate of ELISA (12.8 ％) was lower than that of immunohistochemistry (51.3 ％) (x2 =26.491,P＜0.01).There was statistically significant difference in MG7 Ag expression in gastric cancer with different clinical stages (x2=15.564,P＜0.01).Conclusion This ELISA method might be a non-invasive screening method for population with high risk of gastric cancer.
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Objective To investigate the correlation and significance of cyclooxygenase-2 (COX-2) an d gastric cancer-associated antigen MG7(MG7-Ag) in gastric carcinoma and non- cancerous tissues. Methods Avidin-biotin peroxidase complex immunohistochemical method was adopted to exam ine the expression of COX-2 and MG7-Ag in the gastric mucosa of 100 patients w ith gastric carcinoma. The Helicobacter pylori(H. pylori) infection in gastr ic carcinoma was determined by blue amino toluene stain and modified Giemsa stai n. Results The positive rates of COX-2 and MG7-Ag in gastric carcinoma were 88.0% and 92. 0% respectively. From chronic superficial gastritis, chronic atrophic gastritis, intestinal metaplasia to dysplasia, the expression of COX-2 and MG7-Ag showed a n ascending tendency. Both COX-2 and MG7-Ag were expressed at higher level in cases with lymph nodes or distal organs metastasis than those without (P
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Background and purpose:Through detection of MG7-Ag expression in various types of gastric mucosa biopsy tissues as well as serum,we studied their correlation and its clinical application value in the role of prediction of gastric precancerosis and its clinical significance.Methods:Using 125 samples of gastric mucosa biopsy tissues and corresponding sera(12 samples of normal gastric mucosa,21 samples of superficial gastritis,24 samples of gastric mucosal erosion and ulcer,15 samples of atrophic gastritis,22 samples of dysplasia,31 samples of gastric cancer)MG7-Ag expression in tissues was detected by SP immunohistochemical two-step staining;enzyme-linked immunosorbent assay was used to detect serum content of antigen MG7.Results:No expression of MG7 was detected in 12 samples of normal gastric mucosa and the positive expression rate of MGT-Ag was 93.55% in 31 samples of gastric cancer.In the order of superficial gastritis,gastric mucosal erosion,ulcer,atrophic gastritis,dysplasia and gastric cancer,expression rate of MG7 was increased sequentially(P
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Purpose:In order to deterimine the role of CK7 and other monoclonal antibodies in identification of primary and metastatic malignant ascites due to ovarian carcinoma.Methods:We used immunocytochemical ABC assay to detect the experssions of CK7、MG7、CEA、AB、CK、CK8、EMA、HBME、LCA、VIM monoclonal antibodies in 43 ascitic samples and 22 specimens. Chi square was used for statistic assay with SPSS 8.0 software. Results:One way ANOV assay showed that: (1) Cytokeratin 7 monoclonal antibody can be a very useful mark of primary ovarian carcinoma. (2) MG7 expression in peritoneal cells was significantly different in primary ovarian cancer and metastatic ovarian carcinoma of gastric origin. (3) CEA can be helpful in suggesting the origin of intestine carcinoma or not. (4) Immunohistochemical study of the peritoneal fluids showed results, similar to those in specimens. Conclusions: Immunocytochemical assay in the peritoneal fluids is a helpful diagnostic modality in differentiating between primary and metastatic ascites due to ovarian carcinoma.
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Recently new tumor marker, MG7-Ag has been introduced for screening of gastric cancer. In this study MG7-Ag and CEA in sera of 50 normal healthy Koreans and 48 patients with gastric cancer group were determined to elucidate the clinical usefulness for gastric cancer screening. Commercial Enzyme Immuno Assay kits were used for analysis of above two markers. Sensitivity of MG7-Ag and CEA in patient group were found to be 43.8% and 41.7%, respectively. When the two markers were used combind, sensitivity increased to 62.5%. The concentration of MG7-Ag in patients (9.2+11.7 U/mL) was five times higher than normal control group (1.81+1.63 U/mL). CEA was 31.2+46.5 ng/mL in patients and 2.24+0.98 ng/mL (MeanSD) in normal control group. From this results, combination assay of MG7-Ag and CEA is more useful in clinical laboratories for screening gastric cancer than using one marker.
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Humans , Mass Screening , Stomach NeoplasmsABSTRACT
Localization of MG_7 antigen was investigated in situ by immunohistochemic and immunoclectron microscopic technique in 62 cases of gastrointestinal carcinoma (GICA). The detective rate of MG_7 antigen was 80. 77% in gastric carcinoma (21/26 cases) and 94.44% in colorectal carcinoma (34/36 cases). Its expressing intensity and distribution were associatcd with the differentiation of tumor cells. Most of the poorly differentiated tumors showed much stronger MG_7 antigen expression and cytoplasmic staining, while most of the differentiated tumors showed membranic or mixed staining, mainly distributed in lumen side. Immunoelectronimicroscopic study revealed that MG_7 antigen predominantly existed in lumen margin microvilli, cell membrane and cytoplasm bencath the cell membrane in well differentiated tumor cells, existed in secretory granules, Golgicomplex and endoplasmic reticulum in poorly differetiatd tumor cells. The finding suggest that MG_7 antigen be a kind of secretory substance syntheszed by GICA cells. The mutual relationship between MG_7 antigen localization and tumor cell differetiation as well as its clinical significance were also discussed.
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To explore the expression and function of MG 7 Ag on gastric cancer cell line SGC7901 and its drug resistant subline,SGC7901/VCR, with different resistance index (0 3, 0 7, 1 0) The expression of MG 7 Ag on SGC7901 and SGC7901/VCR cells was determined by immunohistochemical method and flow cytometry (FCM) MTT assay was used to explore the effects of monoclonal antibody MG 7 on drug sensitivity of gastric cancer cells FCM was used to explore the effects of MG 7 on adriamicine accumulation of SGC7901/VCR cells SGC7901/VCR cells exhibit increasing positive staining of MG 7 than SGC7901, according to the increasing resistance index Sensitivity of vincristine of both SGC7901 and SGC7901/VCR cells was reduced after being treated with MG 7 And SGC7901/VCR cells showed decreased adriamicine accumulation when they were incubated with MG 7 Therefore, the conclusion is that MG 7 Ag may be a novel molecule associated with multidrug resistance of gastric cancer, and play an important role in maintaining the drug resistant phenotype of SGC7901/VCR cells.